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To identify roles of RNA binding proteins (RBPs) in the differentiation or survival of antibody secreting plasma cells we performed a CRISPR/Cas9 knockout screen of 1213 mouse RBPs for their ability to affect proliferation and/or survival, and the abundance of differentiated CD138 + cells in vitro. We validated the binding partners CSDE1 and STRAP as well as the m6A binding protein YTHDF2 as promoting the accumulation of CD138 + cells in vitro. We validated the EIF3 subunits EIF3K and EIF3L and components of the CCR4-NOT complex as inhibitors of CD138 + cell accumulation in vitro. In chimeric mouse models YTHDF2-deficient plasma cells failed to accumulate.

Original publication

DOI

10.7554/elife.72313

Type

Journal article

Journal

eLife

Publication Date

22/04/2022

Volume

11

Addresses

Immunology Programme, The Babraham Institute,Babraham Research Campus, Cambridge, United Kingdom.

Keywords

Plasma Cells, Animals, Mice, Carrier Proteins, RNA-Binding Proteins