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A method is described for preparative isoelectric focusing in agarose using low electroendosmotic agarose. Resolution comparable to that seen on analytical polyacrylamide gels is attainable as demonstrated by the isolation of bands with identical idiotypes from the serum of a patient with a monoclonal gammopathy.

Type

Journal article

Journal

J Immunol Methods

Publication Date

1980

Volume

37

Pages

315 - 323

Keywords

Electrophoresis, Agar Gel, Humans, Hypergammaglobulinemia, Immunoglobulin Idiotypes, Isoelectric Focusing, Multiple Sclerosis, Polysaccharides, Sepharose