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The pea chloroplastic outer envelope protein OEP24 is a voltage-dependent channel that can function as a general solute channel in plants. OEP24 is a close functional homologue of VDAC which, in mammalian cells, modulates the permeability of the outer mitochondrial membrane. Here, we describe the production in a one-step reaction of active OEP24 in proteoliposomes or in soluble form using a cell-free expression system. We combine evidence from electrophysiological experiments, biophysical characterization, and biochemical analysis demonstrating that OEP24 is present as a functional channel in liposomes. Thus, production of OEP-containing proteoliposomes may provide a helpful tool for deciphering the role of the OEP family members.

Original publication

DOI

10.1016/j.pep.2009.07.004

Type

Journal article

Journal

Protein Expr Purif

Publication Date

01/2010

Volume

69

Pages

106 - 111

Keywords

Apoptosis, Biological Assay, Biomarkers, Biotechnology, Caspase 9, Cell Survival, Circular Dichroism, Electrophysiological Phenomena, Enzyme Activation, Green Fluorescent Proteins, HCT116 Cells, Humans, Ion Channels, Plant Proteins, Proteolipids, Solubility