Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.5-2.0 swelling/mm(3)), high mechanical strength (2048MPa) and non-pyrogenicity (2.12EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored.

Original publication

DOI

10.1016/j.colsurfb.2015.11.051

Type

Journal article

Journal

Colloids Surf B Biointerfaces

Publication Date

01/03/2016

Volume

139

Pages

1 - 9

Keywords

Acetylated bacterial cellulose, Retinal degenerative diseases, Retinal pigment epithelium, Urinary bladder matrix, Acetylation, Animals, Biomarkers, Cell Adhesion, Cell Culture Techniques, Cell Line, Transformed, Cell Polarity, Cell Proliferation, Cell Survival, Cellulose, Gene Expression, Gluconacetobacter xylinus, Humans, Hydrophobic and Hydrophilic Interactions, Phenotype, Retinal Pigment Epithelium, Swine, Urinary Bladder, Urothelium, Zonula Occludens-1 Protein, cis-trans-Isomerases