Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Neurotrophins are a group of secreted polypeptides, which comprises Nerve Growth Factor (NGF) and Brain-Derived Neurotrophic Factor (BDNF). Each neurotrophin can bind specifically to a tyrosine kinase Trk receptor (TrkA, TrkB or TrkC), while all of the neurotrophins can bind, with similar affinity, to the p75 neurotrophin receptor (p75(NTR)). Experiments on cell viability promotion by BDNF in granule neurons or by NGF in PC12 cells show that neurotrophin-exerted cell viability is neutral sphingomyelinase (nSMase)-dependent, since GW4869 or siRNA knockdown abrogates the protective effects, as well as neurotrophin-induced Akt phosphorylation. Finally, the assessment of nSMase activity promotion drives to the conclusion that neurotrophins can promote cell viability through Trk receptors in a manner depending on basal nSMase but not through SMase activity enhancement.

Original publication

DOI

10.1016/j.febslet.2013.11.032

Type

Journal article

Journal

FEBS Lett

Publication Date

03/01/2014

Volume

588

Pages

167 - 174

Keywords

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, BDNF, Brain-Derived Neurotrophic Factor, CGN, Cer, Ceramide, Granule neuron, MTT, NGF, NT, Nerve Growth Factor, Neurotrophin, PC12, Phosphorylation, SM, SphK, Sphingomyelinase, ceramide, cultured granule neurons, nSMase, neurotrophins, neutral sphingomyelinase, sphingomyelin, sphingosine kinase, Aniline Compounds, Animals, Apoptosis, Benzylidene Compounds, Blotting, Western, Brain-Derived Neurotrophic Factor, Cell Survival, Cells, Cultured, Cerebellum, Microscopy, Fluorescence, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Nerve Growth Factor, Neurons, PC12 Cells, Phosphorylation, Proto-Oncogene Proteins c-akt, RNA Interference, Rats, Receptor, trkA, Receptor, trkB, Receptor, trkC, Sphingomyelin Phosphodiesterase