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Five rat monoclonal antibodies to human uveal melanoma were produced using primary tumor tissue for immunization and screening. These monoclonal antibodies were tested by enzyme-linked immunosorbent assay against 12 uveal melanomas, one breast carcinoma, and peripheral blood lymphocytes from five healthy volunteers. One monoclonal antibody, 4A3, reacted against all the melanomas and none of the controls. On immunoblotting, this monoclonal antibody identified a protein doublet in the molecular weight range 55,000. With these monoclonal antibodies, it was possible to demonstrate significant variation in the antigenic profiles of the uveal melanomas which was not present in the controls.

Type

Journal article

Journal

Invest Ophthalmol Vis Sci

Publication Date

09/1986

Volume

27

Pages

1362 - 1367

Keywords

Animals, Antibodies, Monoclonal, Antigens, Neoplasm, Enzyme-Linked Immunosorbent Assay, Humans, Melanoma, Rats, Rats, Inbred Strains, Uveal Neoplasms