Biochemistry BSc, Imperial College London (2010-13)
Neuroscience MSc, UCL (2013-14)
Clinical Neuroscience DPhil, NDCN, University of Oxford (2015-present)
I am a 3rd year DPhil student under Prof Kevin Talbot, in the Motor Neuron Disease group. The lab focusses on the pathological molecular phenotypes in amyotrophic lateral sclerosis (ALS) or motor neuron disease. I work with a team who are interested in the pathology associated with C9orf72 patient-derived iPS motor neuron models.
I am developing a translating ribosome affinity purification (TRAP) protocol, to pulldown mature motor neuron marker-enriched RNA, I aim to perform RNA Sequencing on RNA derived from C9orf72-patient lines, using CRISPR-corrected lines as a control, to generate translatome profile of the disease. This will potentially allow identification of perturbed pathways, as a direct result of the GGGGCC hexanucleotide expansion in this subtype of ALS.
C9orf72 and RAB7L1 regulate vesicle trafficking in amyotrophic lateral sclerosis and frontotemporal dementia; Aoki Y., Manzano R., Lee Y., Dafinca R., Aoki M., Douglas AGL., Varela MA., Sathyaprakash C., Scaber J., Barbagallo P., Vader P., Mäger I., Ezzat K., Turner MR., Ito N., Gasco S., Ohbayashi N., El Andaloussi S., Takeda S., Fukuda M., Talbot K., Wood MJA.
Development of LNA gapmer oligonucleotide based therapy for FTD/ALS caused by the C9orf72 repeat expansion; Aoki Y., Raquel M., Lee Y., Douglas A., Aoki M., Varela M., Sathyaprakash C., Mutihac R., Talbot K., Wood M.