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Cell-to-cell interactions between the immune and nervous systems are increasingly recognized for their importance in health and disease. Assessment of cellular neuro-immune interactions can be aided by co-culture of two (or more) cells in an in vitro model system that preserves the morphology of neuronal cells. Here we describe methods to investigate the cytotoxic effector functions of natural killer cells on sensory neurons isolated from syngeneic embryonic and adult mice. We present methods for the morphological analysis of axon fragmentation (pruning) and dynamic cell function via live confocal calcium imaging. These techniques can easily be adapted to study interactions between other combinations of immune cell subsets and neuronal populations.

Original publication

DOI

10.1007/978-1-0716-2160-8_18

Type

Chapter

Book title

Natural Killer (NK) Cells.

Publisher

Humana, New York, NY

Publication Date

29/03/2022

Volume

2463

Pages

251 - 268

Total pages

18