Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

<jats:p>Increased dietary consumption of the<jats:italic>n</jats:italic>-3 polyunsaturated fatty acids (PUFA) eicosapentaenoic acid (20:5<jats:italic>n</jats:italic>-3; EPA) and docosahexaenoic acid (22:6<jats:italic>n</jats:italic>-6; DHA) is associated with their incorporation into circulating phospholipid and increased production of lipid peroxide metabolites. The relationship between peripheral blood mononuclear cell (PBMC) function,<jats:italic>n</jats:italic>-3 PUFA intake and antioxidant co-supplementation is poorly defined. We therefore investigated tumour necrosis factor (TNF)-α and interleukin (IL) 6 production by PBMC and phospholipid fatty acid composition in plasma and erythrocytes of healthy male subjects (<jats:italic>n</jats:italic>16) receiving supplemental intakes of 0·3, 1·0 and 2·0 g EPA+DHA/d, as consecutive 4-week courses. All subjects were randomised in a double-blind manner to receive a concurrent antioxidant supplement (200 μg Se, 3 mg Mn, 30 mg D-α-tocopheryl succinate, 90 mg ascorbic acid, 450 μg vitamin A (β-carotene and retinol)) or placebo. There was a positive dose-dependent relationship between dietary<jats:italic>n</jats:italic>-3 PUFA intake and EPA and DHA incorporation into plasma phosphatidylcholine and erythrocyte phosphatidylethanolamine, with a tendency towards a plateau at higher levels of intake. Production of TNF-α and IL-6 by PBMC decreased with increasing<jats:italic>n</jats:italic>-3 PUFA intake but tended towards a ‘U-shaped’ dose response. Both responses appeared to be augmented by antioxidant co-supplementation at intermediate supplementary<jats:italic>n</jats:italic>-3 PUFA intakes. Thus, increased dietary<jats:italic>n</jats:italic>-3 PUFA consumption resulted in defined but contrasting patterns of modulation of phospholipid fatty acid composition and PBMC function, which were further influenced by antioxidant intake.</jats:p>

Original publication

DOI

10.1079/bjn2003892

Type

Journal article

Journal

British Journal of Nutrition

Publisher

Cambridge University Press (CUP)

Publication Date

08/2003

Volume

90

Pages

405 - 412