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We have utilized an oligonucleotide primer from the 3' end of the mouse L1 repeat element for amplification of mouse-specific inter-repeat PCR products from Chinese hamster/mouse somatic cell hybrids. PCR of a Chinese hamster/mouse somatic cell hybrid (96AZ2), containing only mouse chromosome 16, produced a range of mouse-specific bands. Two of the mouse-specific PCR products, of 250 and 580 bp, have been confirmed as originating from mouse chromosome 16 by somatic cell hybrid analysis. Both the 250- and 580-bp PCR products have been sequenced and demonstrate the expected sequence organization. Furthermore, both the 250- and 580-bp markers have been genetically mapped in detail to mouse chromosome 16 by direct hybridization to inter-repeat PCR products of progeny DNAs from Mus domesticus/Mus spretus interspecific backcrosses.

Type

Journal article

Journal

Genomics

Publication Date

11/1991

Volume

11

Pages

679 - 686

Keywords

Animals, Base Sequence, Blotting, Southern, Chromosome Mapping, Cricetinae, Crosses, Genetic, Genetic Linkage, Genetic Markers, Hybrid Cells, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Nucleic Acid Hybridization, Oligodeoxyribonucleotides, Polymerase Chain Reaction, Repetitive Sequences, Nucleic Acid