Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Purpose. Four photorecepior organs -the lateral and parietal eyes, the pineal organ, and the deep brain photoreceptor - are ecogmsed in the lizard Anolis carolinensis, mediating visual and circadian responses to light. Other investigators have identified and sequcnced five different opsins in the Anolis genome. We have studied the expression of these genes in the eye, pinral, and brain. Methods Total RNA was used for the synthesis of cDNA by Supeiscnpt II. Primers were designed lor the specific amplification of rhodopsin, L opsin, rhodopsm-likc M opsin, SI opsin, and S2 opsin, as well as 16S rRNA as a positive control, and used for PCR amplification of the different samples. Results. The different opsins were localized to the dillcrenl photoreceptor organs as follows. PCR pr xlucts were subcloned for sequencing in order to determine specificity: Eye Pineal Brain Rhodopsin + + + L opsin + - - Rhodopsin-like M opsin + - - 51 opsin - + - 52 opsin + + - Conclusions. The detection of rhodopsin mRNA in the rod less Anolis retina confirms immunocytochemical data publ shed by other investigators. The finding of four different photopigments in the eye b consistent with its role in colour vision. Among the circadian photoreceptor systems, we find S opsins in the superficially localized pineal, but not in the deep brain photorecepiors which are unlikely to be exposed to light of these wavelength because of the overlaying tissues. Experiments desiened In confirm our results by RNAse nrntertion assays are in progress.


Journal article


Investigative Ophthalmology and Visual Science

Publication Date