My research focuses on studying the blood-brain-barrier and how different pathologies may affect it. The cerebrovascular field has been growing as an important area of neurodegenerative diseases, with significant phenotypes shown in diseased patients. I am interested as to how diabetes, hyperglycemia, and insulin resistance affects the blood brain barrier, and how that can lead to cognitive impairment. My research currently takes on two aims:
1. Develop an IPSC - brain endothelial cell protocol to model diseases on a dish. Once established, creating an iBBB including pericytes and astrocytes can help identify sources of pathologies within the BBB.
2. Develop a protocol to isolate endothelial and pericytic populations from human post-mortem, fresh frozen tissue, and run transcriptomic analyses on isolated nuclei. This will uncover differentially expressed genes and therapeutic targets to be tested on the IPSC model.
Fine Tuning of Phosphorothioate Inclusion in 2'-O-Methyl Oligonucleotides Contributes to Specific Cell Targeting for Splice-Switching Modulation.
Aoki Y. et al, (2021), Front Physiol, 12