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The aim of this project is to study the effects of disease associated mutations on the N-glycosylation of proteins known to be important to the development and function of the NMJ. Students working on this project will learn to use gene editing, cell culture, molecular biology and protein purification. This project will be co-supervised by Prof David Beeson of NDCN, and will involve collaboration with Dr Stuart Haslam of Imperial College who's group will conduct glycomic and glycoproteomic analysis of purified samples.